Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus.
نویسندگان
چکیده
منابع مشابه
Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus.
We have cloned the Staphylococcus aureus entB gene in Escherichia coli, using pBR322 as the vector plasmid; however, no detectable staphylococcal enterotoxin B (SEB) was produced by the E. coli clones. When the entB gene was placed downstream from the strong lambda phage promoter, PR, SEB was synthesized at readily detectable levels in E. coli. Interestingly, mature SEB was almost exclusively p...
متن کاملmolecular screening of staphylococcal enterotoxin b gene in clinical isolates
objective: the role of staphylococcal enterotoxin b (seb) in food poisoning is well known, however its role in other diseases remains to be explored. the aim of this study is the molecular screening and characterization of the seb gene in clinically isolated strains. materials and methods: in this experimentally study, 300 staphylococcus aureus (s. aureus) strains isolated from clinical samples...
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متن کاملMolecular Screening of Staphylococcal Enterotoxin B Gene in Clinical Isolates
OBJECTIVE The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. MATERIALS AND METHODS In this experimentally study, 300 Staphylococcus aureus (S. aureus) strains isolated from clinical samples...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1985
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.82.17.5850